Effect of miR-705 on osteogenic differentiation of mouse embryo osteoblast precursor cells MC3T3-E1.
- Author:
Xiaohong YANG
1
;
Kun YANG
2
;
Li LIAO
3
;
Yan JIN
4
Author Information
1. Department of Prosthetics, School of Stomatology, Zunyi Medical College, Zunyi 563000, China.
2. Department of Periodontics, School of Stomatology, Zunyi Medical College, Zunyi 563000, China.
3. State Key Laboratory of Military Stomatology, Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi'an 710032, China.
4. State Key Laboratory of Military Stomatology, Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi'an 710032, China. yanjin@fumm.edu.cn.
- Publication Type:Journal Article
- MeSH:
Alkaline Phosphatase;
drug effects;
genetics;
Animals;
Calcification, Physiologic;
drug effects;
genetics;
Cell Differentiation;
drug effects;
genetics;
Core Binding Factor Alpha 1 Subunit;
drug effects;
genetics;
Down-Regulation;
drug effects;
genetics;
Fetal Stem Cells;
Mice;
MicroRNAs;
pharmacology;
Osteoblasts;
drug effects;
Osteocalcin;
drug effects;
genetics;
Osteogenesis;
drug effects;
genetics
- From:
Journal of Zhejiang University. Medical sciences
2016;45(6):575-580
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of miR-705 on osteogenic differentiation of mouse embryo osteoblast precursor (MC3T3-E1) cells.miR-705 mimics, inhibitors and negative control were transfected into MC3T3-E1 cells. Alkaline phosphates (ALP) staining were performed and quantified after 7 days of osteogenic medium induction. The mRNA and protein expression levels of runt-related transcription factor 2 (Runx2) and osteocalcin (OCN) were detected by real-time RT-PCR and Western blot after 14 days of osteogenic induction. Alizarin red staining was performed and quantified in MC3T3-E1 cells after 21 days of osteogenic induction.After 7 days of osteogenic induction, ALP staining showed that overexpression of miR-705 significantly reduced ALP activity, whereas knockdown of miR-705 increased ALP activity (all<0.05). Consistently, after 14 days of osteogenic induction, mRNA and protein expressions of Runx2 and OCN were suppressed by overexpression of miR-705, whereas they were promoted by knockdown of miR-705 (all<0.05). After 21 days of osteogenic induction, alizarin red staining showed that overexpression of miR-705 significantly reduced the formation of mineralized node, the opposite results were found in miR-705 knockdown group (all<0.05).miR-705 can inhibit the osteogenic differentiation of MC3T3-E1 cells.
