High glucose dialysate enhances peritoneal fibrosis through upregulating glucose transporters GLUT1 and SGLT1.
- Author:
Mengqi HONG
1
;
Zhenyu NIE
2
;
Zhengyue CHEN
2
;
Xiongwei YU
2
;
Beiyan BAO
3
Author Information
1. School of Medicine, Ningbo University, Ningbo 315211, China.
2. Division of Nephrology, Ningbo Urology and Nephrology Hospital, School of Medicine, Ningbo University, Ningbo 315192, China.
3. Division of Nephrology, Ningbo Urology and Nephrology Hospital, School of Medicine, Ningbo University, Ningbo 315192, China. baobeiyan2007@sina.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Cells, Cultured;
Connective Tissue Growth Factor;
analysis;
drug effects;
Dialysis Solutions;
adverse effects;
chemistry;
pharmacology;
Gene Expression Regulation;
drug effects;
Glucose;
adverse effects;
pharmacology;
Glucose Transporter Type 1;
analysis;
drug effects;
physiology;
Hemodiafiltration;
adverse effects;
methods;
Humans;
Male;
Peritoneal Dialysis;
adverse effects;
methods;
Peritoneal Fibrosis;
chemically induced;
genetics;
physiopathology;
Peritoneum;
chemistry;
drug effects;
pathology;
Phloretin;
Phlorhizin;
RNA, Messenger;
Rats;
Rats, Sprague-Dawley;
Sodium-Glucose Transporter 1;
analysis;
drug effects;
physiology;
Transforming Growth Factor beta1;
analysis;
drug effects;
Uremia;
chemically induced
- From:
Journal of Zhejiang University. Medical sciences
2016;45(6):598-606
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the role of glucose transporter 1 (GLUT1) and sodium-glucose cotransporter 1 (SGLT1) in high glucose dialysate-induced peritoneal fibrosis.Thirty six male SD rats were randomly divided into 6 groups (6 in each):normal control group, sham operation group, peritoneal dialysis group (PD group), PD+phloretin group (PD+T group), PD+phlorizin group (PD+Z group), PD+phloretin+phlorizin group (PD+T+Z group). Rat model of uraemia was established using 5/6 nephrotomy, and 2.5% dextrose peritoneal dialysis solution was used in peritoneal dialysis. Peritoneal equilibration test was performed 24 h after dialysis to evaluate transport function of peritoneum in rats; HE staining was used to observe the morphology of peritoneal tissue; and immunohistochemistry was used to detect the expression of GLUT1, SGLT1, TGF-β1 and connective tissue growth factor (CTGF) in peritoneum. Human peritoneal microvascular endothelial cells (HPECs) were divided into 5 groups:normal control group, peritoneal dialysis group (PD group), PD+phloretin group (PD+T group), PD+phlorezin group (PD+Z group), and PD+phloretin+phlorezin group (PD+T+Z group). Real time PCR and Western blotting were used to detect mRNA and protein expressions of GLUT1, SGLT1, TGF-β1, CTGF in peritoneal membrane and HPECs., compared with sham operation group, rats in PD group had thickened peritoneum, higher ultrafiltration volume, and the mRNA and protein expressions of GLUT1, SGLT1, CTGF, TGF-β1 were significantly increased (all<0.05); compared with PD group, thickened peritoneum was attenuated, and the mRNA and protein expressions of GLUT1, SGLT1, CTGF, TGF-β1 were significantly decreased in PD+T, PD+Z and PD+T+Z groups (all<0.05). Pearson's correlation analysis showed that the expressions of GLUT1, SGLT1 in peritoneum were positively correlated with the expressions of TGF-β1 and CTGF (all<0.05)., the mRNA and protein expressions of GLUT1, SGLT1, TGF-β1, CTGF were significantly increased in HPECs of peritoneal dialysis group (all<0.05), and those in PD+T, PD+Z, and PD+T+Z groups were decreased (all<0.05). Pearson's correlation analysis showed that the expressions of GLUT1, SGLT1 in HPECs were positively correlated with the expressions of TGF-β1 and CTGF (all<0.05).High glucose peritoneal dialysis fluid may promote peritoneal fibrosis by upregulating the expressions of GLUT1 and SGLT1.
