Myeloid and erythroid hematopoietic transcription factor expression decline after knockdown ofgenes in zebrafish embryos.
- Author:
Shifang HOU
1
,
2
;
Zhihua WANG
3
;
Jun WANG
4
;
Zhixu HE
5
;
Liping SHU
1
;
Author Information
1. Department of Immunology, Guizhou Medical University, Guiyang 550004, China
2. Tissue Engineering and Stem Cell Research Center, Guizhou Medical University, Guiyang 550004, China.
3. Department of Immunology, Guizhou Medical University, Guiyang 550004, China.
4. Clinical Medical Research Center, Guizhou Medical University, Guiyang 550004, China.
5. Tissue Engineering and Stem Cell Research Center, Guizhou Medical University, Guiyang 550004, China
- Publication Type:Journal Article
- MeSH:
Animals;
Down-Regulation;
genetics;
Embryo, Nonmammalian;
physiopathology;
GATA1 Transcription Factor;
genetics;
metabolism;
Gene Knockdown Techniques;
Hematopoiesis;
In Situ Hybridization;
Lamin Type A;
genetics;
physiology;
Proto-Oncogene Proteins;
genetics;
metabolism;
Trans-Activators;
genetics;
metabolism;
Zebrafish;
embryology;
genetics
- From:
Journal of Zhejiang University. Medical sciences
2016;45(6):620-625
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect ofgene down-regulation on early hematopoietic development of zebrafish.Phosphorodiamidate morpholino oligomer (PMO) technology was used to downregulategene expression in Zebrafish. Zebrafish embryos injected phosphorodiamidate morpholino antisense oligonucleotide ofgene mRNA by microinjection at unicellular stage were taken as the experimental group, and those injected meaningless phosphorodiamidate morpholino antisense oligonucleotide were taken as the control. The embryos were collected at 18, 24, 30 and 36 hpf after the fertilization. The real-time fluorescent quantitative PCR (RT-PCR) and whole embryohybridization methods were used to detect the expression of myeloid hematopoietic transcription factorand erythroid hematopoietic transcription factorin zebrafish.RT-PCR showed that the expressions ofanddecreased in the experimental group compared with the control group (all<0.05). Whole embryohybridization showed that the blue-black positive hybridization signals ofandin experimental group were shallow than those in the control group.Myeloid hematopoietic and erythroid hematopoietic of zebrafish are blocked with the downregulation ofgene.
