LC/MS guided approach to discovering nephroprotective substances from Huangkui capsule.
- Author:
Tingting MA
1
;
Yi WANG
2
;
Xiaoqian CHEN
2
;
Xiaoping ZHAO
3
Author Information
1. Zhejiang Chinese Medical University, Hangzhou 310053, China.
2. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
3. Zhejiang Chinese Medical University, Hangzhou 310053, China. zhaoxiaoping@zcmu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Abelmoschus;
chemistry;
drug effects;
Adenosine Triphosphate;
metabolism;
Cell Line, Transformed;
Chromatography, Liquid;
Doxorubicin;
Drugs, Chinese Herbal;
Epithelial Cells;
drug effects;
Flavonoids;
pharmacology;
Kidney Diseases;
chemically induced;
drug therapy;
prevention & control;
Kidney Tubules, Proximal;
drug effects;
Lipid Peroxidation;
drug effects;
Malondialdehyde;
metabolism;
Mass Spectrometry;
Mitochondria;
drug effects;
Oxygen Consumption;
drug effects;
Protective Agents;
chemistry;
pharmacology;
Quercetin;
analogs & derivatives;
pharmacology;
Rutin;
pharmacology
- From:
Journal of Zhejiang University. Medical sciences
2017;46(1):66-73
- CountryChina
- Language:Chinese
-
Abstract:
To discover the nephroprotective substances from Huangkui capsule.The components of Huangkui capsule were isolated by preparative liquid chromatography, and the active components were screened by LC/MS and identified. The adriamycine-injured HK-2 cells were treated with various active components with different concentrations, and the malonaldehyde (MDA) content, adenosine triphosphate (ATP) level and mitochondrial oxygen consumption rate were measured to verify the protective activity of the compounds.Four active components in Huangkui capsule were identified to exert nephroprotective effects. Fifteen flavanoids from these four components were tentatively identified by LC/MS, and hyperin, myricetin, quercetin, rutin and isoquercetin were confirmed. Hyperin, myricetin quercetin and rutin showed dose-dependent protective effects on injured HK-2 cells. Espacially, hyperin significantly reduced MDA content, quercetin and rutin significantly increased ATP level, and myricetin significantly increased mitochondrial oxygen consumption rate.Hyperin, myricetin, querctein and rutin might be the potential nephroprotective compounds in Huangkui capsule, their effects may be related to the inhibition of lipid peroxidation and the alleviation of mitochondrial damage.
