Effects of lncRNA RP11-770J1.3 and TMEM25 expression on paclitaxel resistance in human breast cancer cells.

Yu LI; Yueyue Wang; Haifeng Wang; Lingyu Zhang; Yongxing Ding; Sulian Chen; Qingling Yang; Changjie Chen

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Effects of lncRNA RP11-770J1.3 and TMEM25 expression on paclitaxel resistance in human breast cancer cells.

Author: Yu LI ¹ ; Yueyue WANG ¹ ; Haifeng WANG ¹ ; Lingyu ZHANG ¹ ; Yongxing DING ² ; Sulian CHEN ³ ; Qingling YANG ³ ; Changjie CHEN ⁴
Author Information

1. Clinical Testing and Diagnose Experimental Center, Bengbu Medical College, Bengbu 233000, China.
2. Department of Oncology, the Third People's Hospital of Bengbu, Bengbu 233000, China.
3. Department of Biochemistry & Molecular Biology, Bengbu Medical College, Bengbu 233000, China.
4. Department of Biochemistry & Molecular Biology, Bengbu Medical College, Bengbu 233000, China. tochenchangjie@163.com.
Publication Type:Journal Article
From: Journal of Zhejiang University. Medical sciences 2017;46(4):364-370
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the effects of long non-coding RNA(lncRNA) RP11-770J1.3 and transmembrane protein 25 (TMEM25) on paclitaxel resistance in human breast cancer MCF-7/PR cell line.
METHODSThe expression of lncRNA RP11-770J1.3 and TMEM25 in human breast cancer MCF-7(paclitaxel sensitive) and MCF-7/PR(paclitaxel resistant) cells were detected by quantitative RT-PCR. The synthetic interfering fragments of lncRNA RP11-770J1.3 and TMEM25 were transfected into MCF-7/PR cells. Sulforhodamine B assay was used to detect the sensitivity of MCF-7/PR cells to paclitaxel after interference of lncRNA RP11-770J1.3 and TMEM25. The expression of multidrug-resistance genes and proteins were detected by qRT-PCR and Western blot, respectively.
RESULTSlncRNA RP11-770J1.3 and TMEM25 were highly expressed in MCF-7/PR cells, and were significantly down-regulated after transfection of synthetic interfering fragments. Down-regulation of lncRNA RP11-770J1.3 and TMEM25 enhanced the sensitivity of MCF-7/PR cells to paclitaxel, and inhibited the expression of MRP, BCRP and MDR1/P-gp (all<0.05). Such effects were more significant when lncRNA RP11-770J1.3 and TMEM25 were both down-regulated (all<0.05).
CONCLUSIONSlncRNA RP11-770J1.3 and TMEM25 are highly expressed in MCF-7/PR cells, and the down-regulation of lncRNA RP11-770J1.3 and TMEM25 can enhance paclitaxel sensitivity in MCF-7/PR cells.