The effect of ultra high molecular weight polyethylene particles on macrophage.
- Author:
Yuan-qing MAO
1
;
Zhen-an ZHU
;
Ting-ting TANG
;
Hong-bin LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; In Vitro Techniques; Joint Prosthesis; Macrophages; drug effects; physiology; Mice; NFATC Transcription Factors; biosynthesis; genetics; Phagocytosis; drug effects; Polyethylenes; pharmacology; RNA, Messenger; genetics
- From: Chinese Journal of Surgery 2006;44(12):852-855
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of ultra high molecular weight polyethylene (UHMWPE) particles on macrophages and evaluate the expression of NFAT2, a key transcriptional factor for osteoclast differentiation.
METHODSFrom November 2004 to February 2005, macrophages were co-cultured with UHMWPE particles. When observed at different times, the proliferation activity of macrophages was analyzed by MTT and the expression of calcineurin (CaN) and NFAT2 by immunohistochemical and RT-PCR method respectively.
RESULTSThe macrophages phagocytosed UHMWPE particles in an early time, the expression of CaN and NFAT2 was increased, while the proliferation activity was not enhanced.
CONCLUSIONSUHMWPE particles can stimulate macrophages to phagocytose significantly, and enhance the expression of the transcriptional factor NFAT2.
