A pilot study on the impact of the drug resistance on the radioresistance in human pancreatic cancer cell lines.
- Author:
Ge CHEN
1
;
Yu-pei ZHAO
;
Hua JIANG
;
Jun-chao GUO
;
Li-yang ZHANG
;
Bei-zhan NIU
;
Wei-wei WU
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Cell Cycle; drug effects; Cell Line, Tumor; Deoxycytidine; analogs & derivatives; pharmacology; Doxorubicin; pharmacology; Drug Resistance, Neoplasm; drug effects; genetics; Fluorouracil; pharmacology; Humans; Pancreatic Neoplasms; pathology; Radiation Tolerance; drug effects
- From: Chinese Journal of Surgery 2006;44(13):921-923
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the impact of the drug resistance on the radioresistance in human pancreatic cancer cell lines.
METHODSThree drug resistant pancreatic cancer cell sublines induced by fluorouracil (5-FU), adriamycin (ADM) and gemcitabine respectively, SW1990/FU, SW1990/ADM and SW1990/Gz, were tested for the cell cycle and radio-sensitivity with flow cytometry and clonogenic assay.
RESULTSCompared with SW1990, the cell cycle assay indicated higher G(0)/G(1) period percentage in SW1990/FU and SW1990/Gz, but the G(2)/M period percentage decreased; SW1990/FU had the same while SW1990/Gz had lower S period percentage. SW1990/ADM almost had a similar cell cycle with SW1990. Clonogenic assay showed both SW1990/FU and SW1990/Gz had greater survival fraction (SF(2)) than SW1990, but SW1990/ADM had seemingly similar SF(2) as SW1990.
CONCLUSIONDrug resistant pancreatic cancer cell lines have reduced G(2)/M period percentage and increased radioresistance.
