Percentages of peripheral blood γδ T cells and regulatory T cells and expression of associated cytokines in infants with human cytomegalovirus infection.
- Author:
Ling XU
1
;
Li-Li ZHU
;
Li-Li YE
;
Ling-Jian MENG
;
Wen-Qiang LIU
;
Jun WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cytokines; blood; Cytomegalovirus Infections; immunology; Female; Humans; Infant; Interleukin-17; blood; Male; Receptors, Antigen, T-Cell, gamma-delta; analysis; T-Lymphocytes, Regulatory; immunology; Transforming Growth Factor beta1; blood
- From: Chinese Journal of Contemporary Pediatrics 2018;20(3):204-208
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the percentages of peripheral blood γδ T cells and regulatory T cells (Treg) and the expression of associated cytokines, interleukin 17 (IL-17) and transforming growth factor-β1 (TGF-β1), in infants with human cytomegalovirus (HCMV) infection.
METHODSTwenty-two infants with HCMV infection (HCMV group) and 22 healthy infants who underwent physical examination (control group) were enrolled in this study. The percentages of peripheral blood γδ T cells and Treg cells were determined by flow cytometry. The levels of IL-17 and TGF-β1 in plasma were measured using ELISA.
RESULTSCompared with the control group, the HCMV group had significantly higher percentage of γδ T cells and IL-17 level (P<0.01) and significantly lower percentage of Treg cells and TGF-β1 level (P<0.01). In the HCMV group, the percentage of γδ T cells was negatively correlated with the percentage of Treg cells and TGF-β1 level (P<0.05), but positively correlated with IL-17 level (P<0.05); the percentage of Treg cells was positively correlated with TGF-β1 level (P<0.05), but negatively correlated with IL-17 level (P<0.05); there was no correlation between IL-17 level and TGF-β1 level (P>0.05).
CONCLUSIONSThere is an imbalance between γδ T cells and Treg cells in the peripheral blood of infants with HCMV infection, and γδ T cells may be involved in the secretion of IL-17.
