Biological effects of nano-hydroxyapatite/polyamide 66 on the osteoblast.

Author: Ling YE ¹ ; Qin SU ; Xue-dong ZHOU
Author Information

1. Key Lab. of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
Publication Type:Journal Article
MeSH: Durapatite; pharmacology; Nylons; pharmacology; Osteoblasts; drug effects; Osteocalcin; metabolism; RNA, Messenger; metabolism
From: West China Journal of Stomatology 2005;23(2):142-144
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo evaluate the biological effects of nano-hydroxyapatite/polyamide 66(nHA-PA66) on the growth and activity of osteoblast.
METHODSMTT assay was used to determine the growth of osteoblast, enzymatic measure was used to determine the activity of ALP and quantitative RT-PCR (QRT-PCR) to evaluate the changes of osteoclacin mRNA expression in osteoblasts treated by DMEM eluate of nHA-PA66.
RESULTSOsteoblasts of different test groups demonstrated relative proliferation rate ranging from 98% - 106% without dose-dependent effect. The ALP activity and osteocalcin mRNA expression were similar in test and control groups (P > 0.05).
CONCLUSIONnHA-PA66 has no negative effects on the osteoblast and its osteoblast-compatibility is proved.