Osteogenic differentiation of synovial mesenchymal stem cells in vitro.
- Author:
Jian LI
1
;
Xing LONG
;
Fan ZHU
;
Xue-chao YANG
Author Information
- Publication Type:Journal Article
- MeSH: Ascorbic Acid; Cell Differentiation; Dexamethasone; Glycerophosphates; Humans; Mesenchymal Stromal Cells; Osteoblasts; Osteogenesis; Osteopontin; metabolism; RNA, Messenger; metabolism
- From: West China Journal of Stomatology 2005;23(2):145-151
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the potential of synovial mesenchymal stem cells (SMSC) in osteogenic differentiation.
METHODSSMSC were obtained by limited dilution method and expanded to culture in 25-milliliter flasks. The attached cells were treated with inductive medium containing dexamethasone, glycerophosphate and vitamin C at 3rd passage SMSC. The mineralized nodule was stained by Von Kossa method. The expression of ALP and osteopontin were detected by histochemical, immunohistological staining technique, respectively, while the expression of cbfa1 mRNA by RT-PCR.
RESULTSPure SMSC which were of spindle shape and star shape, uniform in size, could be induced to pleomorphism osteoblast in vitro, which were intensive positive in ALP and osteopontin. The expression of cbfa1 mRNA were also verified by RT-PCR and the polygonal cells formed nodular structure at 4 weeks. All these were coincident with the characters of osteoblast.
CONCLUSIONSMSC can be purified and induced into osteoblast in vitro.
