Expression and secretion of human tumor necrosis factor gene transfected on human embryo myoblasts.
- Author:
Zhen-nan GAO
1
;
Jia-rang CAO
;
Wei-dong TIAN
;
Sheng-wei LI
;
Lei LIU
;
Chun-hua FU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Genetic Therapy; Humans; Myoblasts; Neoplasm Invasiveness; prevention & control; Plasmids; Tongue Neoplasms; therapy; Transfection; Tumor Necrosis Factor-alpha; metabolism
- From: West China Journal of Stomatology 2005;23(2):170-172
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe human tumor necrosis factor-alpha (hTNF-alpha) expression and secreting level of human embryo myoblasts transfected by hTNF-alpha gene.
METHODSHuman embryo myoblasts were transfected with shuttle plasmid pSV23SHTNF containing hTNF-alpha gene by cationic liposomes DOSPER. The control group was only given equivalent liposomes except plasmid. After culturing for 24, 48, 72 and 96 hours, hTNF-alpha expression level of human embryo myoblasts was observed with immunocytochemistry staining, and hTNF-alpha secreting of human embryo myoblasts was analyzed by ELISA.
RESULTSAfter transfected by hTNF-alpha gene for 24, 48, 72 and 96 hours, the human embryo myoblasts displayed significant secretion of hTNF-alpha in the cultural supernatant (P < 0.05), and overexpression in cytoplasma and cell membrane.
CONCLUSIONTransfection of hTNF-alpha gene to human myoblasts made myoblasts secrete high concentration of hTNF-alpha, implying it is feasible that transfecting muscle cells surrounding tongue carcinoma lesion with hTNF-alpha gene can prevent tongue carcinoma from intruding into deeper muscle tissue.
