Effect of hypoxia on the expression of urokinase-type plasminogen activator in human tongue squamous cell carcinoma cells (Tca8113).

Author: Sheng-zhi WANG ¹ ; Xin-hua LIANG ; Zhou CHENG ; Hong-guang BI ; Zu-yi MAO ; Do-zhang WANG
Author Information

1. Dept. of Oral and Maxillofacial Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai 264000, China.
Publication Type:Journal Article
MeSH: Carcinoma, Squamous Cell; Humans; Mouth Neoplasms; Neoplasm Invasiveness; Tongue Neoplasms; Urokinase-Type Plasminogen Activator
From: West China Journal of Stomatology 2005;23(3):211-213
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo observe the expression of urokinase-type plasminogen activator (uPA) in human tongue squamous carcinoma cells (Tca8113) under hypoxia, in order to explore the relation between hypoxia and invasion and metastasis of oral cancer.
METHODSUnder different hypoxic times(0, 3, 6, 12, 24 h), the expression of uPA protein was examined quantitatively using immunohistochemical technique (IH) and flow cytometry (FCM).
RESULTSHypoxia promoted the expression of uPA. The longer hypoxic time was, the higher expression of uPA was.
CONCLUSIONHypoxia can promote invasion and metastasis of oral squamous carcinoma through stimulating the expression of uPA.