OBJECTIVETo observe the different mRNA levels of Candida albicans ALS gene family between planktonic and biofilm-grown cells.

METHODSATCC 90038 and a wild strain of Candida albicans, biofilm models in vitro were formed on glass slides. After 48 hours' incubation, the biofilm-grown cells were harvested. Half-quantification of ALS1 and ALS4 mRNA was based on the amplification by one-step RT-PCR.

RESULTSThe amounts of ALS1 and ALS4 mRNA of the wild strain in biofilm increased comparing with planktonic cells, while ATCC 90038 didn't.

CONCLUSIONThe members of ALS gene family may play important roles in the course of Candida albicans biofilm formation.