Influence of vascular cell co-cultured with osteoblast in vitro on ability of osteoblast.
- Author:
Jian ZHANG
1
;
Min HU
;
Wen-yi ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; Animals; Coculture Techniques; In Vitro Techniques; Osteoblasts; Rabbits
- From: West China Journal of Stomatology 2005;23(4):325-328
- CountryChina
- Language:Chinese
-
Abstract:
UNLABELLEDOBJECTIVE; The purpose of this study was to observe the influence on proliferation of rabbit osteoblast (ROB) of rabbit vascular endothelial cell(RVEC) co-cultured with rabbit osteoblast (ROB).
METHODSRabbit osteoblasts and rabbit vascular endothelial cells in ratio of 1:0, 1:1, 2:1, 4:1 were selected to co-culture. The function of osteoblasts was assessed by alkaline phosphatase (ALP) activity and osteocalcin (OC) assay.
RESULTSThere was good compatibility when osteoblasts and vascular endothelial cells were co-cultured. The activity of ALP and OC in the group of ROB and RVEC co-cultured in ratio of 2:1 was higher than that of the other three groups.
CONCLUSIONIn direct co-culture system in vitro, RVEC can improve activity of ROB.
