Chemical constituents of leaf of Eucommia ulmoides.
- Author:
Fang YANG
;
Zheng-Gang YUE
;
Xin WANG
;
Xiu-Peng ZHANG
;
Jiang CHAI
;
Jiu-Cheng CUI
;
Xiao-Mei SONG
;
Qi-Bing MEI
- Publication Type:Journal Article
- MeSH:
Antioxidants;
chemistry;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
chemistry;
Eucommiaceae;
chemistry;
Magnetic Resonance Spectroscopy;
Molecular Structure;
Plant Leaves;
chemistry
- From:
China Journal of Chinese Materia Medica
2014;39(8):1445-1449
- CountryChina
- Language:Chinese
-
Abstract:
Ten compounds were isolated from the leaf of Eucommia ulmoides by means of recrystallization and chromatographic techniques such as D-101 macroporous resin, MCI resin, ODS gel, Sephadex LH-20 and Rp-HPLC. Their structures were identified by NMR spectral analyses as kaempferide 3-O-beta-D-glucoside (1), quercetin-3-O-beta-D-glucoside (2), quercetin (3), quercetin-3-O-beta-D-xylosyl-(1-->2)-beta-D-galactoside (4), kaempferol-3-O-alpha-L-rhamnosyl-(1-->6)-beta-D-glucoside (5), (2S,3S)-taxifolin 3-O-beta-D-glucoside (6) ,4-hydroxy cinnamic acid (7), (+)-cycloolivil (8), pinoresinol beta-D-glucoside (9), squalene (10). Among them compounds 1,5-7,10 were isolated from the Eucommia genus for the first time. In the DPPH free radical scavenging assay, compound 2 exhibited significant activity (IC50 13.7 micromol x L(-1)), compared with vitamin C (IC50 59.9 micromol x L(-1)); compounds 1, 3 and 9 showed moderate activity (IC50 161,137, 214 micromol x L(-1)), compared with 2,6-di-tert-butyl-4-methylphenol (IC50 236 micromol x L(-1)); compound 4 and 6 showed weak activity (IC50 264, 299 micromol x L(-1)).
