Determination of amino acids in Galli Gigerii endothelium corneum by HPLC with pre-column derivatization.
- Author:
Kun LIANG
;
Dan ZHANG
;
Ji SHI
;
Hong HU
;
Yan-Feng XIU
;
Tian-Zhu JIA
- Publication Type:Journal Article
- MeSH:
Amino Acids;
analysis;
Animals;
Chickens;
Chromatography, High Pressure Liquid;
Chromatography, Reverse-Phase;
Endothelium;
chemistry;
Gizzard, Avian;
chemistry
- From:
China Journal of Chinese Materia Medica
2014;39(8):1463-1467
- CountryChina
- Language:Chinese
-
Abstract:
Hydrolytic amino acids were extracted by acid hydrolysis method, then derivatized with phenyl isothiocyanate (PITC). And the samples were analysed by HPLC on an Ultimate Prime C18 (4.6 mm x 250 mm, 5 microm) column with gradient elution of 0.1 mol x L(-1) sodium acetate buffer solution (adjusted to pH 6. 5)-acetonitrile (93:7) (A) and acetonitrile-water (8:2) (B) at a flow rate of 1.0 mL x min(-1). Column temperature was 40 degrees C and the detected wavelength was 254 nm. Amino acids derivative solution remained stable in 36 hours. The response was linear for 16 amino acids with a correlation coefficient r > 0.999 5. The average recoveries were 98.01% -101.8%. The method is reliable with good accuracy and repeatability, which is useful for the determination of amino acids in Galli Gigerii Endothelium Corneum.
