Preliminary study on pharmacodynamic evaluation method of Houpo formula particles.
- Author:
Lu MA
;
Li-Jie SHAO
;
Fang TANG
- Publication Type:Journal Article
- MeSH:
Animals;
Chemistry, Pharmaceutical;
methods;
Colitis, Ulcerative;
drug therapy;
metabolism;
Dosage Forms;
Drug Evaluation, Preclinical;
Drugs, Chinese Herbal;
adverse effects;
chemistry;
pharmacokinetics;
Humans;
Magnolia;
chemistry;
Male;
Rats;
Rats, Wistar;
Substance P;
metabolism;
Vasoactive Intestinal Peptide;
metabolism
- From:
China Journal of Chinese Materia Medica
2014;39(8):1490-1494
- CountryChina
- Language:Chinese
-
Abstract:
To discuss the feasibility of the pharmacodynamic evaluation method for traditional Chinese medicine (TCM) formula particles, with traditional decoction for reference and the intervention of Magnoliae Officinalis Cortex in rats with ulcerative colitis (UC). First of all, the similarity of traditional Magnoliae Officinalis Cortex decoction and formula particles of different manufacturers was defined by using the IR fingerprint. The UC rat model was established and given Houpo formula particles of different doses and manufacturers, with the decoction for reference, in order to observe disease activity index (DAI), colon mucosa damage index (CMDI), pathologic changes, nitric oxide (NO), endothdin (ET), substance P, vasoactive intestinal peptide (VIP). Their intervention effects on UC rats were compared to study the difference between Sanjiu and Tianjiang Houpo formula particles, in order to demonstrate the feasibility of the pharmacodynamic evaluation method for Houpo formula particles. According to the results, Houpo formula particles showed similar pharmacodynamic actions with the traditional decoction. The pharmacodynamic comparison of Houpo formula particles of different manufacturers showed no statistical significance. The experiment showed that on the basis of the TCM compounds, a prescription dismantlement study was conducted to define target points of various drugs. The traditional decoction was selected for reference in the comparison of corresponding formula particles for their pharmacodynamic equivalence. This method could avoid controversies about single or combined boiling of formula particles, and give objective comments on the pharmacodynamic effect of the formula particles. The method is proved to be feasible.
