Experimental study on anti-tumor effect of xihuang pill and its immune clearance function.
- Author:
Jie MA
;
Yi-Yao WANG
;
Wei YANG
;
Shuo GUAN
;
Chang-Qian ZENG
;
Wen-Bin GAO
;
Wen-Bo LIANG
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents; administration & dosage; B7-1 Antigen; genetics; immunology; Breast Neoplasms; drug therapy; genetics; immunology; CD4-Positive T-Lymphocytes; drug effects; immunology; Drugs, Chinese Herbal; administration & dosage; Female; Flow Cytometry; Humans; Immune System; drug effects; Immunologic Factors; administration & dosage; Interferon-gamma; genetics; immunology; Interleukin-2; genetics; immunology; Rats; Rats, Wistar; Tumor Burden; drug effects
- From: China Journal of Chinese Materia Medica 2014;39(8):1499-1501
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo discuss the anti-tumor effect of Xihuang pill on tumor-bearing rats and its effect on the immune clearance function of tumor-bearing organisms.
METHODWalker256 tumor cells were adopted to establish the tumor-bearing rat model. The rats were randomly divided into five groups: the normal control group, the model control group, the lentinan group and Xihuang pill low dose, middle dose and high dose groups, with 10 rats in each group, and continuously treated and given drugs for 14 d after modeling. Blood and tumors were collected from abdominal aorta to calculate the tumor inhibition rate. The content of CD3+, CD4+, CD8+ T cells and adhesion molecule B7-1 (CD80) in peripheral blood were detected by flow cytometry (FCM). The expressions of IL-2 and IFN-gamma in were determined by ELISA.
RESULTThe tumor inhibition rate of the Xihuang pill high dose group was 33. 1 percent. Compared with the model group, the Xihuang pill large dose group showed significantly low IL-2, IFN-gamma, CD3+, CD4+, B7-1 in peripheral blood, with statistical significance in their differences (P < 0.05).
CONCLUSIONXihuang pill could show its anti-tumor effect by enhancing the immune clearance function and increasing IL-2, IFN-gamma, CD3+ T, CD4+ T, B7-1 in peripheral blood.