Simultaneous determination of 4 alkaloids and a flavonoid in Picrasmae Ramulus et Folium by RP-HPLC.
- Author:
Chong WU
;
Gui-Hua DENG
;
Chao-Zhan LIN
;
Chen-Chen ZHU
- Publication Type:Journal Article
- MeSH:
Alkaloids;
analysis;
Calibration;
Carbolines;
analysis;
Chromatography, High Pressure Liquid;
methods;
Chromatography, Reverse-Phase;
methods;
Flavonoids;
analysis;
Indole Alkaloids;
analysis;
Picrasma;
chemistry;
Plant Leaves;
chemistry;
Plant Stems;
chemistry;
Pterocarpans;
analysis;
Reproducibility of Results
- From:
China Journal of Chinese Materia Medica
2014;39(9):1656-1659
- CountryChina
- Language:Chinese
-
Abstract:
A RP-HPLC method was developed to evaluate the quality of Picrasmae Ramulus et Folium by simultaneous determination of five constituents including 1-hydroxymethyl-beta-carboline (1), 1-methoxicabony-beta-carboline (2), 4-methoxy-5-hydroxy-canthin-6-one (3), 4, 5-dimethoxy-canthin-6-one (4) and maackiain (5) in Picrasmae Ramulus et Folium. The samples were separated on a Kromasil RP-C18 (4.6 mm x 250 mm, 5 microm) column eluted with acetonitrile and 0.1% phosphoric acid as mobile phases in gradient mode. The detection wavelength was set at 254 nm. The calibration curves and linearity of the above five standards were determined as (1) Y = 6 525.6X + 37.25 (0.009-1.780 microg, r = 0.996 8), (2) Y = 3 662.3X + 41.55 (0.005-0.920 microg, r = 0.999 5), (3) Y = 3763.1X + 146.87 (0.015-3.060 microg, r = 0.999 0), (4) Y = 2 174.1X + 21.52 (0.003-0.620 microg, r = 0.999 5), and (5) Y = 276.25X + 7.65 (0.010-1.960 microg, r = 0.998 9), respectively. The method is simple and repeatable, and can be used for the quality assessment of Picrasmae Ramulus et Folium.
