Effect of Antrodia cinnamomea on gene expression related to aortal endothelial injury in rats with hyperlipidemia.
- Author:
Jie QI
;
Yun TAO
;
Jun ZHANG
;
Jian FU
- Publication Type:Journal Article
- MeSH: Animals; Antrodia; chemistry; Aorta; drug effects; metabolism; ultrastructure; Atherosclerosis; blood; genetics; prevention & control; Biological Products; pharmacology; Cholesterol; blood; Cholesterol, HDL; blood; Cholesterol, LDL; blood; Endothelium, Vascular; drug effects; metabolism; pathology; Enzyme-Linked Immunosorbent Assay; Gene Expression; drug effects; Hyperlipidemias; blood; genetics; prevention & control; Lipoproteins, LDL; blood; Male; Malondialdehyde; blood; Microscopy, Electron; NF-kappa B; blood; genetics; metabolism; Random Allocation; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Scavenger Receptors, Class E; blood; genetics; metabolism; Superoxide Dismutase; blood; Triglycerides; blood; p38 Mitogen-Activated Protein Kinases; blood; genetics; metabolism
- From: China Journal of Chinese Materia Medica 2014;39(9):1670-1674
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Antrodia cinnamomea on gene expression related to aortal endothelial injury of rats with hyperlipidemia.
METHODFifty SD rats were randomly divided into five groups: the normal control group (NG), the model group (MG), the antrodia cinnamomea groups of low, middle and high doses (AC-LG, AC-MG, AC-HG, 250, 500, 1 000 mg x kg(-1)). The rats were fed with high-fat diets to establish the hyperlipidemia model. After the drug administration for 10 weeks, their serum lipid, SOD, MDA and ox-LDL, LOX-1, P38 MAPK and NF-kappaB mRNA and protein expression were respectively determined, and the aortal endothelial injury was observed under electron microscope.
RESULTIn the model group, the contents of TC, TG and LDL-C significant increased (P < 0.01), whereas the content of HDL-C significant decreased (P < 0.01). Compared with the model group, both the AC-M group and the AC-H group showed reduction in endothelial injury and significant decrease in the content of TC, TG and LDL-C (P < 0.05 or P < 0.01). The content of HDL-C increased, but with no significant difference. SOD activity in serum remarkably increased (P < 0.05 or P < 0.01), MDA and ox-LDL levels dramatically decreased (P < 0.05 or P < 0.01).
CONCLUSIONA. cinnamomea can alleviate endothelial lipid injury by inhibiting the expressions of LOX-1, P38MAPK and NF-kappaB in aorta and better protect aortal endothelial cells from oxidative lipid injury.