Absorption and transportation of calycosin in Astragali Radix by using Caco-2 monolayer model.
- Author:
Zhou LE
;
Xiao-Li ZHAO
;
Liu-Qing DI
- Publication Type:Journal Article
- MeSH:
Absorption;
Biological Transport;
Caco-2 Cells;
Chromatography, High Pressure Liquid;
Culture Media, Conditioned;
chemistry;
Drug Stability;
Drugs, Chinese Herbal;
analysis;
pharmacokinetics;
Humans;
Hydrogen-Ion Concentration;
Isoflavones;
analysis;
pharmacokinetics;
Models, Biological
- From:
China Journal of Chinese Materia Medica
2014;39(9):1709-1713
- CountryChina
- Language:Chinese
-
Abstract:
Flavonoids are a class of important active ingredients in traditional Chinese medicine, pharmacological activity and in vivo process is the focus of research in recent years. Calycosin is the main active ingredients of flavonoids in Astragali Radix, recent studies indicate that it has many kinds of pharmacological activity, but the absorption and transport characteristics in vivo is unclear. The experiment using Caco-2 cell model, with apigenin as internal standard substance, using the method for the determination of drug concentration by HPLC, were studied at different concentrations and absorption transport characteristics of respectively adding different types of protein inhibitors. Data were analyzed by Q test, the results show that low, middle, high concentration of P(app)(BL-AP)/ P(app)(AP-BL) = 1.38 < 1.5, respectively adding different types of protein inhibitors, compared with the control group of P(app)(BL-AP)/ P(app)(AP-BL), there were no significant differences. Calycosin absorption may mainly passive transport, also involved in active transport mechanism, the transport may not be affected by the P-protein, MRP2 protein, SGLT protein.
