Determination of gamma-aminobutyric acid in aerial part of Panax notoginseng by HPLC.
- Author:
Jing-Jing YANG
;
Ying LIU
;
Xiu-Ming CUI
;
Yuan QU
;
Lu-Qi HUANG
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Flowers;
chemistry;
Panax notoginseng;
chemistry;
Plant Extracts;
analysis;
Plant Leaves;
chemistry;
gamma-Aminobutyric Acid;
analysis
- From:
China Journal of Chinese Materia Medica
2014;39(4):606-609
- CountryChina
- Language:Chinese
-
Abstract:
The content of gamma-aminobutyric acid in the aerial part of Panax notoginseng in different productive area was determined by using high performance liquid chromatography. HPLC analysis was made on a C18 (4.6 mm x 250 mm,5 microm) with acetonitrile and water containing 4.1 g x L(-1) sodium acetate as mobile phase. The detection wavelength was 254 nm. The HPLC method showed good linearity within the range of 0.01 - 1.03 g x L(-1). The average recovery of GABA in the stems and leaves of P. notoginseng and the flowers of P. notoginseng was 101.7% (RSD 1.1%, n = 3) and 97.3% (RSD 0.38%, n = 3), respectively. The contents of GABA in the samples of different productive areas were not significant different, and the average contents of GABA in the stems and leaves of P. notoginseng and the flowers of P. notoginseng were 0.49% and 0.53%. This method was simple and reliable, and it was suitable for the determination of gamma-aminobutyric acid in the aerial part of P. notoginseng.
