Study on inhibitory effects and mechanism of lipophilic components in Salvia miltiorrhiza on angiogenesis in vitro.
- Author:
Xue-Mei FAN
;
Gui-Xiang REN
;
Qiong-Lin LIANG
;
Yi-Ming WANG
;
Guo-An LUO
- Publication Type:Journal Article
- MeSH:
Angiogenesis Inhibitors;
chemistry;
pharmacology;
Cell Movement;
drug effects;
Cell Proliferation;
drug effects;
Drugs, Chinese Herbal;
chemistry;
pharmacology;
Human Umbilical Vein Endothelial Cells;
cytology;
drug effects;
metabolism;
Humans;
Matrix Metalloproteinase 9;
genetics;
metabolism;
Salvia miltiorrhiza;
chemistry;
Vascular Endothelial Growth Factor A;
genetics;
metabolism
- From:
China Journal of Chinese Materia Medica
2014;39(4):744-747
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the human umbilical vein endothelial cell model was used to study the regulating effect of lipophilic components in Salvia miltiorrhiza on angiogenesis, and explore its possible mechanism. The cell model was established to determine the effect of lipophilic components in S. miltiorrhiza on the proliferative activity and migration capacity of endothelial cells. Then the realtime fluorescence quantification PCR technology was applied to detect the changes in the gene expressions of angiogenesis-related cytokines VEGF-A, VEGF-C and MMP-9. The results showed that 5 mg x L(-1) lipophilic components in S. miltiorrhiza could inhibit the proliferation and migration of endothelial cells, and reduce the expression of VEGF-A and MMP-9 genes. It indicated that lipophilic components in S. miltiorrhiza may inhibit the proliferation and migration of endothelial cells by inhibiting the expression of VEGF-A and MMP-9 genes, so as to show the inhibitory effect on angiogenesis.
