Differential gene expression of liver carcinoma cell after transfection of by ARL-1 with microarray.
- Author:
Da-nian TANG
1
;
Yong-guo LI
;
Jun-min WEI
;
Xiang-dong YANG
;
Min-wei ZHU
;
Pei WU
;
Xiong-ying MIAO
Author Information
- Publication Type:Journal Article
- MeSH: Aldehyde Reductase; genetics; Drug Resistance, Neoplasm; Gene Expression Profiling; Humans; Liver Neoplasms; drug therapy; genetics; Oligonucleotide Array Sequence Analysis; Transfection
- From: Chinese Journal of Surgery 2003;41(3):180-182
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the difference of the gene expression profile and to identify the different expression after transfection of the ARL-1 gene.
METHODSThe cDNA probes were synthesized from total RNA of study group and control group, which was differentially hybridized to cDNA chips and confirmed by a gene specific semiquantitative reverse transcription polymerase chain reaction (RT-PCR).
RESULTSSix kinds of gene expression were increased and 9 kinds of gene expression were decreased. The findings were correlated with protein metabolism, signal pathway, metastasis, and drug resistance.
CONCLUSIONScDNA chips showed that gene expression profile of liver carcinoma cell was changed after transfection of the ARL-1 gene. It is a useful method in understanding the mechanism of drug resistance.
