Preferential expression of cartilage oligomeric matrix protein in degenerating acinar cells in chronic pancreatitis and in chronic pancreatitis-like lesions.
- Author:
Quan LIAO
1
;
Yu-pei ZHAO
;
Jorg KLEEFF
;
Yi XIAO
;
Arthur ZIMMERMANN
;
Markus W BUCHLER
;
Helmut FRIESS
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Northern; Blotting, Western; Cartilage Oligomeric Matrix Protein; Dimerization; Extracellular Matrix Proteins; chemistry; genetics; metabolism; Glycoproteins; chemistry; genetics; metabolism; Humans; Immunohistochemistry; In Situ Hybridization; Matrilin Proteins; Pancreas; metabolism; pathology; Pancreatic Neoplasms; metabolism; pathology; Pancreatitis, Chronic; metabolism; pathology; RNA, Messenger; genetics; metabolism
- From: Chinese Journal of Surgery 2003;41(5):328-331
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo study cartilage oligomeric matrix protein (COMP) mRNA and protein expression in normal pancreas, chronic pancreatitis (CP), and pancreatic cancer tissues.
METHODSTissues from 15 cases of normal pancreas, 14 cases of chronic pancreatitis and 14 cases of pancreatic cancer were analyzed by Northern blot, Western blot, in situ hybridization and immunohistochemistry.
RESULTSCOMP mRNA signals and immunoreactivity were strongly present in the cytoplasm of degenerating acinar cells in CP tissues as well as in CP-like lesions in pancreatic cancer tissues. In contrast, COMP expression was weak to absent in the cytoplasm of cancer cells in pancreatic cancer tissues, and in ductal cells and islet cells in normal pancreatic tissues.
CONCLUSIONCOMP is preferentially expressed in degenerating acinar cells in CP and in CP-like areas in pancreatic cancer, suggesting a potential role of this molecular in acinar cell dysfunction in CP.
