Construction of eucaryotic expression plasmid carrying the BMP7 gene and expression in mesenchymal stem cells.
- Author:
Shu-xun HOU
1
;
Da-ming SUN
;
Gui-xin DU
;
Yi-gang TONG
;
Xiao-bing FU
Author Information
- Publication Type:Journal Article
- MeSH: Bone Morphogenetic Protein 7; Bone Morphogenetic Proteins; genetics; Genetic Therapy; Humans; Mesenchymal Stromal Cells; metabolism; Plasmids; Polymerase Chain Reaction; Tissue Engineering; Transforming Growth Factor beta
- From: Chinese Journal of Surgery 2003;41(6):445-448
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo construct an eucaryotic expression plasmid carrying the BMP7 gene and express in MSCs.
METHODSThe BMP7 gene was cloned into the eucaryotic expression vector pcDNA3.1. At the same time, mesenchymal stem cells (MSCs) were isolated and cultured in vitro. The plasmid carrying the BMP7 gene was transfected into MSCs.
RESULTSPCR and digesting demonstrated that the eucaryotic expression plasmid -pcDNA-BMP7 was obtained. RT-PCR and immunohistochemical methods showed that the BMP7 gene was expressed in MSCs.
CONCLUSIONConstruction of an eucaryotic expression plasmid carrying BMP7 gene and expression in MSCs provide a sound basis for gene therapy using the BMP7 gene and the ideal seeds for tissue engineering.
