Study on repairing injured middle brain by Schwann cells transplantation.
- Author:
Ye LIN
1
;
Qing-san ZHU
;
Fei YIN
;
Li GUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Transplantation; GAP-43 Protein; analysis; Immunohistochemistry; Mesencephalon; injuries; metabolism; surgery; Rats; Rats, Wistar; Schwann Cells; metabolism; transplantation; Time Factors
- From: Chinese Journal of Surgery 2004;42(3):187-189
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the ability of grafted Schwann cells to promote restore of injured neurons in rat.
METHODSSchwann cells labeled by BrdU in vitro transplanted into rat middle brain area prior to injure with electric needle stimulus. Immunohistochemistry and image analyzer were used to investigate the expression of BrdU and GAP-43 as well as quantitative analysis respectively.
RESULTSBrdU positive cells could be identified for up to 8 months and the number increased about 15%, which mainly migrated toward injured ipsilateral cortex. The GAP-43 expression reached its peak one month after transplantation and was significant compared with control group (P < 0.05).
CONCLUSIONThe transplantation of Schwann cells could promote the restoration of injured neurons.
