The expression of Cyclin D1 modulated by somatotropin on human pancreas cancer cell lines Bxpc-3.
- Author:
Fei LI
1
;
Da-chuan LIU
;
Jia-bang SUN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cell Division; drug effects; Cell Line, Tumor; Cyclin D1; biosynthesis; Dose-Response Relationship, Drug; Growth Hormone; pharmacology; Humans; Immunohistochemistry; In Situ Nick-End Labeling; Pancreatic Neoplasms; metabolism; pathology; Time Factors
- From: Chinese Journal of Surgery 2004;42(7):432-434
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the growth effect of somatostapin on human pancreas cancer lines Bxpc-3.
METHODSThe Bxpc-3 pancreas cancer cells were treated with Somatotropin. The cells hyperplasia were detected by MTT and were observed apoptosis cells determinated quantitatively by TUNEL, quantify immune fluoresence double marked the proliferation cells and apoptosis cells, the expression of Cyclin D1 detected by immunohistochemical.
RESULTSThe growth effect of pancrea cancer cells were limited by 10(-7) M, 10(-8) M, 10(-9) M Somatotropin on 2 day. The limited effect was decreased from 3 day. The cells proliferation were increased by somotostapin on 4day to 5day. The relationship between the expression of Cyclin D1 and apoptosis was negative correlation and the cells hyperplasia was positive correlation in Bxpc-3 cell line.
CONCLUSIONFrom the cell study we knew the expression of Cyclin D1 reflected the prolefiration of pancreas cancer cells.
