Experiment and study of endothelialization of prostheses with bone marrow CD34+ cells.
- Author:
Hong-kun ZHANG
1
;
Shu-sen ZHENG
;
Zhong-gao WANG
;
Ming LI
;
Wei JIN
;
Hua FENG
;
Ping SHAN
;
Hai-ge ZHAO
;
Xu-dong CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, CD34; metabolism; Blood Vessel Prosthesis; Blood Vessel Prosthesis Implantation; Bone Marrow; blood supply; Bone Marrow Cells; drug effects; metabolism; Cell Differentiation; drug effects; Dogs; Endothelial Cells; drug effects; ultrastructure; Female; Flow Cytometry; Male; Microscopy, Electron; Vascular Endothelial Growth Factor A; pharmacology
- From: Chinese Journal of Surgery 2004;42(7):435-438
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo exploration the endothelialization of prostheses with bone marrow CD(34)(+) cells.
METHODSCD(34)(+) cells were isolated from bone marrow of carine by an immune magnetic cell sorting system and induced into endothelial cells with VEGF. Seeding the cells to PTFE prostheses which implanted the abdominal aorta artery (AAA) and inferior vena cava (IVC).
RESULTSThe isolated cells from bone marrow were CD(34)(+) by flow cytometer which could differentiate into endothelial cells in vascular endothelial growth factor (VEGF). The endothelial cells were identified by immunostaining and transmission electron microscope. The obliteration rate of the seeding grafts implanting AAA was 0%, the stenosis rate 12.5%; the obliteration rate implanting IVC 12.5%, the stenosis rate 25%.
CONCLUSIONCD(34)(+) cells were isolated from bone marrow by an immune magnetic cell sorting system and were able to be induced into endothelial cells with VEGF in vitro. PTFE prostheses seeding CD(34)(+) cells have the ideal endothelialization and patency.
