Effects of substance P on granulation tissue fibroblasts proliferation and expression of basic fibroblast growth factor mRNA.
- Author:
Wei JIANG
1
;
Zheng-guo WANG
;
Xi-nan LAI
;
Jin-ming ZHU
;
Pei-fang ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Division; drug effects; Dose-Response Relationship, Drug; Fibroblast Growth Factor 2; genetics; Fibroblasts; cytology; drug effects; metabolism; Gene Expression; drug effects; Granulation Tissue; cytology; drug effects; metabolism; Male; RNA, Messenger; genetics; metabolism; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction; Substance P; pharmacology
- From: Chinese Journal of Surgery 2004;42(6):366-368
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the proliferation-promoting effect of sensory neuropeptide substance P (SP) on the cultured granulation tissue fibroblasts in vitro and its regulative effect on the gene expression of basic fibroblast growth factor (bFGF) mRNA.
METHODSThe proliferation-promoting effect of cultured granulation tissue fibroblasts was observed by means of MTT; the regulative effect of SP on gene expression of fibroblast bFGF by RT-PCR. The time and dose-efficiency relations were also observed.
RESULTSThere was a significant proliferation-promoting effect of SP on the cultured granulation tissue fibroblasts in vitro in a remarkable dose-dependent fashion. However, bFGF antibody only partly exerted its inhibitive effect. SP could induce the bFGF mRNA expression of the fibroblasts at the 3rd and 6th hour (P < 0.01). SP could promote the bFGF mRNA expression of the fibroblasts in the concentration of 10(-9) - 10(-5) mol/L and peaked in the concentration of 10(-7) mol/L.
CONCLUSIONSSP has a significant proliferation-promoting effect on the granulation tissue fibroblasts, which is correlated with SP inducing bFGF mRNA expression of fibroblasts.
