The study on correlation of c-myc gene expression with vascular smooth muscle cell proliferation in patients with portal hypertension.
- Author:
Jin-hua ZENG
1
;
Zhen YANG
;
Jun XU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Cell Proliferation; Female; Gene Expression; Genes, myc; genetics; Humans; Hypertension, Portal; genetics; pathology; In Vitro Techniques; Male; Middle Aged; Muscle, Smooth, Vascular; cytology; metabolism; Myocytes, Smooth Muscle; cytology; Proliferating Cell Nuclear Antigen; metabolism; RNA, Messenger; genetics; Splenic Vein; cytology; metabolism
- From: Chinese Journal of Surgery 2004;42(9):543-545
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate c-myc proto-oncogene expression and the relationship of PCNA protein expression of extrahepatic vascular smooth muscle cell in patients with portal hypertension and normal vessels.
METHODSRT-PCR was used to demonstrate the expression of c-myc mRNA and immuno-chemistry strain was performed to detect the expression of PCNA protein in splenic veins of 28 patients with portal hypertension and 12 normal vessels.
RESULTSThe straining of PCNA protein was (29.8 +/- 4.7)% in splenic veins with portal hypertension, Normal vessels did not detect PCNA protein expression (P < 0.01); RT-PCR showed that the expression of c-myc mRNA in PCNA-positive control and in negative control of splenic veins with portal hypertension were (7.61 +/- 1.04)% and (3.82 +/- 0.92)%, respectively. There ws different between two groups (P < 0.01) and significant different (P < 0.01) when compared with (1.01 +/- 0.21)% in normal vessels.
CONCLUSIONSThe c-myc was immediate-early gene when it modulated proliferation of vascular smooth muscle cell. Hemodynamic disturbance of portal vein system activate the proto-oncogene of smooth muscle cells in splenic vein of patients with portal hypertension, promoting the proliferation, migrating and phenotypic change and resulting in vascular remodelling of splenic veins.
