Experimental study on islet cells in rats under condition of three-dimensional microgravity.
- Author:
Chun SONG
1
;
Xiu-qing DUAN
;
Yi ZHOU
;
Xi LI
;
Li-ou HAN
;
Ping XU
;
Chun-fang SONG
;
Lian-hong JIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bioreactors; Cell Culture Techniques; methods; Cell Survival; Cells, Cultured; Insulin; secretion; Islets of Langerhans; cytology; secretion; Male; Rats; Rats, Wistar; Weightlessness Simulation
- From: Chinese Journal of Surgery 2004;42(9):559-561
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the survival rate and secretory function of islet cells in rats under condition of three-dimensional microgravity.
METHODSIsolated islet cells were assigned to flask-cultured or bioreactor-cultured. Survival rate of islets cultured for days 3, 7, 14 in stationary flasks or microgravity bioreactors was measured by AO-PI double-staining. Cultured islets were identified by dithizone (DTZ) staining, and insulin contents of different culture liquids were measured by radioimmunoassay.
RESULTSPancreatic islets stained nacarat with DTZ were easily visualized. When islet cells were cultured for 7 days and 14 days, survival rate of bioreactor-cultured islets was (0.9000 +/- 0.0107)% and 0.8038% +/- 0.0092% and higher than flask-cultured islets (P < 0.01). Insulin level of bioreactor-cultured islets is (70.875 +/- 0.31) m micro /L on the cultured 7 days while flask-cultured islets is (41.246 +/- 0.35) m micro /L. There was statistically significant difference of insulin production between the two groups (P < 0.01). Bioreactor-cultured islet contents were higher than flask-cultured on the cultured 14, 21 and 30 days (P < 0.01).
CONCLUSIONSIslet cells survival rate and secretory function revealed that bioreactor-cultured islets functioned better compared with flask-cultured islets.
