Determination of polysaccharides content of Gentiana farreri from different producing areas based on anthrone-sulfuric acid method.
- Author:
Li LIN
;
Yan WANG
;
Fu-Xing WANG
;
Jia-Yan HE
;
Hang-Lin ZHANG
- Publication Type:Journal Article
- MeSH:
Anthracenes;
chemistry;
Chemistry Techniques, Analytical;
methods;
Gentiana;
chemistry;
growth & development;
Geography;
Linear Models;
Polysaccharides;
analysis;
Reproducibility of Results;
Sulfuric Acids;
chemistry;
Time Factors
- From:
China Journal of Chinese Materia Medica
2014;39(14):2774-2776
- CountryChina
- Language:Chinese
-
Abstract:
Study a method for the detemination of the content of polysaccharides in Gentiana farreri, and analysis of the content of polysaccharides from different producing areas. The results showed that using the anthrone-sulfuric acid method, simple operation, accurate result. Sample was measured at 620 nm absorbance after anthrone-sulfuric acid color, at this wavelength, solution absorption and glucose showed a good linear relationship; The linearity was in the range of 0.01-0.07 g x L(-1) (r = 0.996 7). The recovery rate was 99.41%, with RSD of 2.0%. Considering the experimental conditions, to determine the solid-liquid ratio 1:60, extracting time 50 min, concentration of ethanol 80%. The mass fraction of polysaccharides was the highest to reached 0.743% in G. farreri from Gansu Xiahe. This experiment has laid a good foundation for further study on G. farreri.
