Mechanism exploration on synthesis of secondary metabolites in Sorbus aucuparia cell cultures treated with yeast extract.
- Author:
Lei HUANG
;
Wen-Juan XIAO
;
Guang YANG
;
Ge MO
;
Shu-Fang LIN
;
Zhi-Gang WU
;
Lan-Ping GUO
- Publication Type:Journal Article
- MeSH:
Cell Culture Techniques;
instrumentation;
methods;
Culture Media;
chemistry;
metabolism;
Saccharomyces cerevisiae;
chemistry;
Secondary Metabolism;
Sorbus;
growth & development;
metabolism
- From:
China Journal of Chinese Materia Medica
2014;39(11):2019-2023
- CountryChina
- Language:Chinese
-
Abstract:
Suspension cultures cell of Sorbus aucuparia (SASC) was used as materials, the changes of physiological and biochemical indexes of SASC after treatment with yeast extract (YE) were detected, and the synthetic mechanism of secondary metabolites in SASC treated with YE was preliminarily explored. The results were as follows: under the assay conditions, SASC was induced to synthesize five biphenyl compounds, and these compounds content changed differently with induction time prolonging; YE treatment inhibited cell growth, the culture medium pH was gradually reduced after treatment; water-soluble protein content showed a trend of slow decline, which was significantly increased in YE treatment group (YE group) compared with the control group (CK group), the maximum relative content was 147.76% in contrast with CK group; both YE group and CK group were extracellular Ca2+ flow influx, but the YE group flow was significantly slow than CK group. The results indicate that YE induced the cells in a stress state, which was not conducive to the growth of cells and forced the cells to synthesize biphenyl compounds against external stress; water-soluble protein may serve as intracellular enzymes involved in the synthesis of compounds regulation; Ca2+ may as signal molecule mediate cell signal transduction respond to YE stress.
