Study on protective effect of total saponins of Panax japonicus on LPS-induced RAW264. 7 cell inflammation through NF-kappaB pathway.
- Author:
Yan-Wen DAI
;
Ding YUAN
;
Jing-Zhi WAN
;
Chang-Cheng ZHANG
;
Chao-Qi LIU
;
Ting WANG
- Publication Type:Journal Article
- MeSH: Animals; Anti-Inflammatory Agents; pharmacology; Drugs, Chinese Herbal; pharmacology; Humans; Inflammation; drug therapy; genetics; immunology; Interleukin-1beta; genetics; immunology; Lipopolysaccharides; adverse effects; Macrophages; drug effects; immunology; Mice; NF-kappa B; genetics; immunology; Nitric Oxide; immunology; Nitric Oxide Synthase Type II; genetics; immunology; Panax; chemistry; Protective Agents; pharmacology; Saponins; pharmacology
- From: China Journal of Chinese Materia Medica 2014;39(11):2076-2080
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the anti-inflammatory effect of total saponins of Panax japonicus on LPS-induced RAW264. 7 macrophages.
METHODThe effect of total saponins of P. japonicus of different concentrations on RAW264. 7 cell viability was determined with the MTT method. The NO kit assay was adopted to detect the NO release of total saponins of P. japonicus to LPS-induced RAW264. 7 cells. The enzyme linked immunosorbent assay (ELISA) was used to detect the secretion of tumor necrosis factor-alpha (TNF-alpha) and interleukin 1-beta (IL-1beta). The reverse transeriptase-polymerase chain reaction (RT-PCR) was used to determine the expression of inducible nitric oxide synthase (iNOS) ,TNF-alpha,IL-1beta. The protein expression of nuclear transcription factor-kappaB p65 (NF-kappaB p65) was tested by Western blot.
RESULTThe safe medication range of total saponins of P. japonicus was less than 80 mg x L(-1). Compared with the LPS model group, total saponins of P. japonicus high, middle and low dose groups (0.1, 1, 10, 40 mg x L(-1)) could significantly reduce the secretion of NO, TNF-alpha, IL-1beta of LPS-induced RAW264. 7 cells, and inhibit the expressions of iNOS, TNF-alpha and IL-1beta mRNA and the protein expression of NF-kappaB p65.
CONCLUSIONThis study preliminarily proves the protective effect of total saponins of P. japonicus on LPS-induced RAW264.7 macrophages. Its action mechanism may be related to NF-kappaB signal pathway.