Establishment of red fluorescent protein orthotopic transplantation nude mice metastasis model of pancreatic cancer and whole-body fluorescent imaging.
- Author:
Ze-qian YU
1
;
Jia-hua ZHOU
;
Ke-tao TAO
;
Liang HU
;
Jie ZHENG
;
De-tong YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Female; Luminescent Proteins; Male; Mice; Mice, Nude; Neoplasm Metastasis; Pancreas; pathology; Pancreatic Neoplasms; pathology; Xenograft Model Antitumor Assays
- From: Chinese Journal of Surgery 2009;47(14):1092-1095
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a stable high red fluorescent protein (RFP)-expressing orthotopic transplantation nude mice spontaneous metastasis model of pancreatic cancer.
METHODSStable high RFP-expressing cells SW1990-RFP were injected subcutaneously into mice to establish subcutaneous implantation model. Fluorescent tumor piece from subcutaneous was transplanted into the body of the pancreas to establish surgical orthotopic implantation model. The growth of primary tumor, metastasis and micrometastasis were assessed by whole-body fluorescence imaging system.
RESULTSTwelve RFP orthotopic transplantation nude mice metastasis models of pancreatic cancer were established successfully, the percentage of success rate was 100%. RFP-labeled pancreatic cancer growth could be monitored in real time way. The micrometastasis of primary lesions were detected in early stage with whole-body fluorescence imaging system.
CONCLUSIONSThe RFP orthotopic transplantation nude mice metastasis model of pancreatic cancer is stable and reliable, and can be observed dynamically in vitro in a noninvasive way, with much higher sensitivity and specificity.
