The effect of PTC1 transfection on the growth and apoptosis of human pulmonary adenocarcinoma cell A549.
- Author:
Qi-bin SONG
1
;
Wei-guo HU
;
Yi YAO
;
Wei-xing WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; metabolism; pathology; Apoptosis; Cell Line, Tumor; Cell Proliferation; Gene Expression Regulation, Neoplastic; Genetic Vectors; Humans; Lung Neoplasms; metabolism; pathology; Patched Receptors; Plasmids; genetics; Receptor, Epidermal Growth Factor; metabolism; Receptors, Cell Surface; genetics; metabolism; Transfection
- From: Chinese Journal of Surgery 2009;47(17):1340-1342
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of the tumor suppressor gene PTC on the growth inhibition and down-regulation of epidermal growth factor receptors (EGFR) in pulmonary adenocarcinoma cell A549.
METHODSPulmonary adenocarcinoma cell A549 were divided into wild type group, mutant type group, blank group and control group. They were transfected with wild-type PTC1 plasmids, mutant-PTC1 plasmids and blank plasmids, respectively. After transfection, the cell growth curve was drown every day for a week. The expression of PTC1 and EGFR were detected by western blot. Flow cytometry was used to analyze apoptosis and cell cycle of the transfected cells.
RESULTSAfter transfected with wild-type PTC1, the growth rates of A549 cells were slow down, but the other groups of cells had no change. Compared with the control group, the expression of EGFR were down-regulated. The apoptosis rates in wild type group was 24.5%, and the mutant type group was 8.3% (P < 0.01). But the apoptosis rate of blank group has no change.
CONCLUSIONWild-type PTC1 could induce apoptosis and inhibitory effect on A549 cells.
