Effects of intracoronary transplantation of autologous bone marrow mononuclear cells or endothelial progenitor cells in mini-swine model of myocardial ischemia-reperfusion.
- Author:
Chong-jian LI
1
;
Run-lin GAO
;
Yue-jin YANG
;
Feng-huan HU
;
Wei-xian YANG
;
Lai-feng SONG
;
Ying-mao RUAN
;
Shu-bin QIAO
;
Ji-lin CHEN
;
Xue-wen QIN
;
Zai-jia CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; cytology; Bone Marrow Transplantation; Coronary Circulation; Disease Models, Animal; Endothelial Cells; cytology; Female; Male; Myocardial Reperfusion Injury; therapy; Stem Cells; cytology; Swine; Swine, Miniature; Transplantation, Autologous
- From: Chinese Journal of Cardiology 2007;35(10):936-939
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the effects of intracoronary transplantation of autologous bone marrow mononuclear cells (BM-MNC) or peripheral endothelial progenitor cells (EPC) in mini-swine model of myocardial ischemia-reperfusion.
METHODSThe Mini-swine acute myocardial infarction and reperfusion model was created with 90 min occlusion of the left anterior descending coronary artery followed by reperfusion and the animals were then divided into BM-MNC group (3.54 x 10(8) +/- 0.90 x 10(8), n = 9), EPC group (1.16 x 10(7) +/- 1.07 x 10(7), n = 7) and control group (saline, n = 7). Echocardiography, hemodynamic measurements and myocardium infarction size were evaluated before and 4 weeks after intracoronary cell transplantations.
RESULTSThe net decrease from baseline to 4 weeks after transplantation of left ventricular ejection fraction (LVEF), left ventricular end systolic pressure, cardiac output and +dp/dt(max) were significantly attenuated post BM-MNC and EPC therapy compared to control group (all P < 0.05) and were similar between BM-MNC and EPC groups. Transplantation of BM-MNC and EPC also significantly decreased myocardial infarction size compared to control group.
CONCLUSIONAutologous intracoronary transplantation of BM-MNC or EPC in this model equally improved cardiac systolic function and reduced infarction area.
