Effect of fibrinogen,fibrin and fibrin (ogen) degradation products on the tissue plasminogen activator and plasminogen activator inhibitor-1 expressions of vasculat endothelial cells in coculture system
10.3321/j.issn:0253-3758.2008.01.015
- VernacularTitle:纤维蛋白原、纤维蛋白及其降解产物对共培养血管内皮细胞纤溶活性的影响
- Author:
Yong-Jun CAO
1
;
Yan-Hua WU
;
Chun-Feng LIU
Author Information
1. 苏州大学附属第二医院
- Keywords:
Arteriosclerosis;
Fibrin;
Fibrinogen;
Fibrin fibrinogen degradation products
- From:
Chinese Journal of Cardiology
2008;36(1):62-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of fibrinogen(Fg),fibrin(Fb)and fibrin(ogen) degradation products(FDPs)on tissue plasminogen activator(tPA)and plasminogen activator inhibitor-1 (PAI-1)expressions of human umbilical vein endothelial cells(HUVECs)in coculture system.Methods Fg,Fb and FDPs at various concentrations(0,0.5,1.5,3.0,4.5 and 6.0 g/L)were added to the transwell cocuhure system of HUVECs and smooth muscle cells (SMCs)for 24 hours.The expressions of tPA and PAI-1 at mRNA level were examined by RT-PCR and tPA and PAI-1 protein and activity were detected by ELISA and substrate chromogenic assays.Results tPA expression was not affected bv Fg.Fg at concentrations between 3.0-4.5 g/L significantly enhanced the mRNA expression,protein content and activity of PAI-1,while expression of PAI-1 was significantly inhibited by Fg at concentration of 6.0 g/L.Fb at concentrations between 3.0-4.5 g/L significantly up-regulated mRNA expression,increased protein content and downregulated activity of tPA.Fb(1.5-4.5 g/L)also enhanced the mRNA expression,increased protein content and activity of PAI-1.FDPs at concentrations 3.0-6.0 g/L down-regulated the expression of tPA and FDPs at concentrations 1.5-6.0 g/L significantly enhanced PAI-1 mRNA expression.Conclnsion Fg,Fb and FDPs play important roles in the pathogenesis of atherosclerosis by modulating the expression of tPA and PAI-1 of endothelial and SMCs.
