N-acetylcysteine promoted aging through adjusting expression of cell cycle related protein in neonatal SD rat cardiomyocytes
10.3321/j.issn:0253-3758.2008.02.012
- VernacularTitle:N-乙酰半胱氨酸通过调控细胞周期蛋白加重乳鼠心肌细胞的衰老
- Author:
Feng-Xiang ZHANG
1
;
Ming-Long CHEN
;
Bing YANG
;
Hong-Wu CHEN
;
Ke-Jiang CAO
Author Information
1. 南京医科大学附属第一医院
- Keywords:
Myocytes;
cardiac;
Acetylcysteine;
Cell aging
- From:
Chinese Journal of Cardiology
2008;36(2):146-150
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of N-acetylcysteine(NAC)on aging in neonatal SD rat cardiomyocytes and explore related mechanisms.Methods Cultured eardiomyoeytes were randomized assigned to 6 groups:1-day,5-day,10一day,1-day+NAC(1 mmol/L),5-day+NAC(1 mmol/L)and 10-day+NAC(1 mmol/L).Flow eytometry was used to examine cell cycle.Real-time quantitative PCR and Western blot were used to determine mRNA and protein expression of p16INK4a,p21WAF1 and Rb gene.β-galactosidase staining kit was used to investigate β-galaetosidase activity.Results Numbers of cardiomyocytes resided in G0/G1 phase were significantly higher in the group of 5-day+NAC and 10-day+NAC compared with 5-day,10-day,respectively(P＜0.05).The mRNA and protein expression of p16INK4a and p21WAF1 were also significantly higher in the group of 5-day+NAC and 10-day+NAC compared with 5-day,10-day,respectively(P＜0.05 or P＜0.01).The mRNA and protein expression of Rb was significantly lower in the group of 5-day+NAC and 10-day+NAC compared with 5-day,10-day, respectively(P＜0.01).β-galactosidase activity was not affected by NAC in the 1-day+NAC group but was significantly higher in 5-day+NAC and 10-day+NAC groups compared with the 5-day,10-day groups(all P＜0.05).Conclusion NAC could promote aging through upregulating the expression of p16INK4a and p21 WAF1 and inhibiting Rb phosphorylation in neonatal SD rat cardiomyoeytes.
