Role of alkaloid sinomenine in chronic rejection in the rat heart transplantation model
10.3321/j.issn:0253-3758.2008.02.016
- VernacularTitle:青藤碱对大鼠心脏移植慢性排斥反应抑制作用的实验研究
- Author:
Guang-Quan XU
1
;
Xiang-Yuan JIN
;
Guo-Cai YANG
;
Ce ZHANG
;
Da-Zhong LIU
;
Qiu-Ming XIA
Author Information
1. 哈尔滨医科大学附属第二医院
- Keywords:
Heart transplantation;
Graft rejection;
Sinomenine
- From:
Chinese Journal of Cardiology
2008;36(2):167-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the possible role of alkaloid sinomenine(SIN)on chronic rejection in rat heart transplantation model.Methods After a brief course of cyclosporine A(CsA),DA recipients of PVG hearts were treated with placebo,SIN,CsA,or a combination of both drugs.Grafts were analyzed morphometrically and by immuno-histochemistry.Expressions of basic fibroblast growth factor (bFGF),vascular endothelial growth factor(VEGF),and endothelin 1(ET-1)were assessed by reverse transcription-polymemse chain reaction.Results Cardiac grafts of SIN-treated rats showed a mild degree of vasculopathy compared with untreated rats or CsA-treated recipients.Degree of vasculopathy was significantly reduced in rats treated with combined SIN and CsA than rats receiving either drug alone.Treatment with SIN alone did not affect gene expressions of bFGF,VEGF,and ET-1 while expressions of bFGF,VEGF,and ET-1 were significantly reduced by combined treatment with SIN and CsA.Conclusion These results demonstrated a potential value of SIN,in combination with low-dose CsA to attenuate the vasculopathy in this rat model of chronic cardiac allograft rejection.
