Expression of FLICE-inhibitory protein in synovial tissue and its association with synovial inflammation in juvenile idiopathic arthritis.
- Author:
Feng-Xia WU
1
;
Li-Jun WU
;
Xiong-Yan LUO
;
Ming-Hui YANG
;
Zhong TANG
;
Chuan-Mei XIE
;
Jing-Guo ZHOU
;
Jian-Long GUAN
;
Guo-Hua YUAN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Arthritis, Juvenile; metabolism; pathology; CASP8 and FADD-Like Apoptosis Regulating Protein; genetics; metabolism; Caspase 8; metabolism; Child; Female; Humans; Inflammation; metabolism; pathology; Male; Protein Isoforms; genetics; metabolism; Synovial Membrane; cytology; metabolism; pathology
- From: Chinese Medical Sciences Journal 2010;25(1):20-26
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo examine the expression of FLICE-inhibitory protein (FLIP) in juvenile idiopathic arthritis (JIA) and analyze its correlation with synovial inflammation.
METHODSThe expression of FLIP was assessed in 11 JIA and 3 normal synovial tissue samples by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. The cell types expressing FLIP were further characterized, and the correlation of FLIP expression with the degree of synovial inflammation, as well as the activity of caspase 8 was then analyzed.
RESULTSRT-PCR revealed the expression of FLIP mRNA in all 11 JIA samples, but not in 3 normal synovial tissues. In JIA, FLIP expression could be found in both the lining and sublining layers, mainly in the macrophage-like cells. Moreover, the expression of FLIP in JIA synovial tissues was positively correlated with the degree of synovial inflammation (r = 0.563, P < 0.05).
CONCLUSIONThe expression of antiapoptotic FLIP in JIA synovial tissue and its correlation to accumulation of inflammatory cells in synovial tissue suggests that FLIP potentially extends the lifespan of synovial cells and thus contributes to the progression of joint destruction.
