Antagomir dependent microRNA-205 reduction enhances adhesion ability of human corneal epithelial keratinocytes.
- Author:
Jun LI
1
;
Hua BAI
;
Yong ZHU
;
Xiao-yan WANG
;
Fang WANG
;
Jun-wu ZHANG
;
Robert M LAVKER
;
Jia YU
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; Cell Adhesion; genetics; Cells, Cultured; Epithelium, Corneal; cytology; Humans; Keratinocytes; cytology; MicroRNAs; antagonists & inhibitors; Oligonucleotide Probes
- From: Chinese Medical Sciences Journal 2010;25(2):65-70
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effect of microRNA-205 reduction by antagomirs on adhesion ability of normal human corneal epithelial keratinocytes (NHCEKs).
METHODSAntagomir-205, complementary and inhibitory to microRNA-205, was used to suppress endogenous microRNA-205 in NHCEKs. The adhesion ability of treated NHCEKs was then assessed by cell adhesion assay. Immunoblot and immunohistochemistry were conducted to determine the level of two focal adhesion-related proteins, focal adhesion kinase (FAK) and paxillin (Pax). Phalloidin staining was performed to measure the level of filamentous actin in antagomir-treated NHCEKs.
RESULTSAntagomir-205 markedly reduced the level of microRNA-205 in NHCEKs and significantly enhanced adhesion ability of NHCEKs (P<0.01). Further protein analysis validated that inhibition of microRNA-205 increased the number of phosphorylated FAK and phosphorylated Pax, and decreased filamentous actin.
CONCLUSIONOur findings suggest that microRNA-205 has down-regulating effect on cell motility in NHCEKs.
