Gaussia luciferase reporter assay for assessment of gene delivery systems in vivo.
- Author:
Feng CHEN
1
;
Zhen XU
;
Jiang LU
;
Xiang LÜ
;
Wen-li MU
;
Ya-jun WANG
;
De-pei LIU
;
Chih-chuan LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Arecaceae; enzymology; Cell Line; Gene Transfer Techniques; Genes, Reporter; Humans; Luciferases; genetics; Mice
- From: Chinese Medical Sciences Journal 2010;25(2):95-99
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo develop an alternative method for assessment of gene delivery systems in vivo.
METHODSMouse primary spleen lymphocytes were genetically modified in vitro by a retroviral vector harboring a Gaussia luciferase (Gluc) expression cassette. After implantation of these cells into recipient mice, the expression of Gluc was detected in whole blood or plasma collected.
RESULTSAs little as 10 muL whole blood drawn from the recipient mice could guarantee prompt reading of Gluc activity with a luminometer. And the reading was found in good correlation with the number of genetically modified spleen lymphocytes implanted to the mice.
CONCLUSIONSGluc may be useful as an in vivo reporter for gene therapy researches, and Gluc blood assay could provide an alternative method for assessment of gene delivery systems in vivo.
