Liquid chromatography-tandem mass spectrometry for analysis of acylcarnitines in dried blood specimens collected at autopsy from neonatal intensive care unit.
- Author:
Wen-jun TU
1
;
Fang DAI
;
Xin-yu WANG
;
James Jian HO
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Metabolism, Inborn Errors; blood; diagnosis; Autopsy; Carnitine; blood; Chromatography, Liquid; methods; Female; Humans; Infant, Newborn; Intensive Care Units, Neonatal; Male; Tandem Mass Spectrometry; methods
- From: Chinese Medical Sciences Journal 2010;25(2):109-114
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the feasibility of analyzing acylcarnitine in dry filter-paper blood spots by liquid chromatography-tandem mass spectrometry (LC-MS/MS) which could be applied to detect inborn errors of metabolism in neonates.
METHODSWe obtained filter-paper blood from 26 dead infants from a neonatal intensive care unit (NICU) between October 1, 2008 and September 30, 2009. Acylcarnitine and amino acid profiles were obtained with LC-MS/MS. Four infants underwent routine autopsy. The postmortem blood specimens were compared with newborn blood specimens, and with specimens obtained from older infants with metabolic disorders.
RESULTSOf all the 26 patients, 5 (19.2%) were diagnosed as having different kinds of diseases: 3 with methylmalonic acidemia (the concentration of C3, and the ratio of C3/C16, C3/C2 increased), 1 with maple syrup urine disease (the concentration of leucine and isoleucine increased), and 1 with isovaleric acidemia (the concentration of C5 increased).
CONCLUSIONSPostmortem metabolic test can explain infant deaths and provide estimates of deaths attributable to inborn errors of metabolism in NICU. LC-MS/MS is suitable for analysis of postmortem specimens and can be considered for routine application in NICU autopsy.
