Culture and identification of human amniotic mesenchymal stem cells.

Author: Huo SHUANG-ZHI ¹ ; Shi PING ; Pang XI-NING
Author Information

1. Department of Stem Cells and Regenerative Medicine, China Medical University, Shenyang 110001, China.
Publication Type:Journal Article
MeSH: Amnion; cytology; Cell Culture Techniques; Flow Cytometry; Humans; Immunohistochemistry; Mesenchymal Stromal Cells; cytology
From: Chinese Medical Sciences Journal 2010;25(4):211-214
CountryChina
Language:English
Abstract:
OBJECTIVETo establish the method of isolation, purification, and identification of human amniotic mesenchymal stem cells (hAMSCs).
METHODShAMSCs were isolated from human amniotic membrane by trypsin-collagenase digestion, and cultured in Dulbecco's modified Eagle's medium/F12 medium supplemented with 10% fetal bovine serum. Phenotypic characteristics of these cells were analyzed by means of immunocytochemistry and flow cytometry.
RESULTSThe cells successfully isolated from human amniotic membrane expressed representative mesenchymal cell surface markers CD44, CD90, and vimentin, but not CD45.
CONCLUSIONSThis study establishes a potential method for isolation of hAMSCs from human amnion,in vitro culture, and identification. The isolated cells show phenotypic characteristics of mesenchymal stem cells.