α-GalCer administration after allogeneic bone marrow transplantation improves immune reconstitution in mice.
- Author:
Jing-hua LIU
1
;
Jing-Hua LIU
;
Li-ping DOU
;
Li-Ping DOU
;
Li-xin WANG
;
Li-Xin WANG
;
Li-li WANG
;
Li-Li WANG
;
Fan ZHOU
;
Li YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; B-Lymphocytes; drug effects; immunology; Bone Marrow Transplantation; immunology; Female; Galactosylceramides; pharmacology; Graft vs Host Disease; immunology; Hematopoietic Stem Cells; drug effects; Lymphocyte Activation; drug effects; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; T-Lymphocytes; drug effects; immunology; Transplantation, Homologous
- From: Chinese Medical Sciences Journal 2011;26(2):91-97
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the effect of α- galactosyleramide( α-GalCer ) on immune reconstitution under acute graft-versus-host disease (aGVHD).
METHODSBALB/c mice were transplanted wit hallogeneic C57BL/6 bone marrow cells and splenocytes (both 1×10(7))after receiving lethal total-body irradiation. α-GalCer (100 ug/kg) or vehicle (dimethylsulfoxide) was administered intraperitoneally immediately after transplantation. The effects of α-GalCer on immune reconstitution,proliferation of T cells and B cells, hematopoiesis,and thymic microenvironment were assessed.
RESULTSThe α-GalCer group exhibited higher percentages of CD3(+),CD4(+), CD8(+), B220(+), CD40(+), and CD86(+)cells compared with the vehicle group . The number of colony forming unit per 1000 CD34(+) cells in the α-GalCer group was higher than in the vehicle group ( P=0.0012).In vitro proliferation assays showed that the α-GalCer group had higher percentages of CD3(+), CD4(+), CD8(+),and B220(+) cells compared with the vehicle group. As for the results of in vivo proliferation assays, the numbers of CD3(+), CD4(+), CD8(+), and B220(+)cells were higher in the α-GalCer group than in the normal group ,especially the number of B220(+) cells ( P=0.007).Significant difference was not found in thymocyte count between the α-GalCer group and the vehicle group, nor in the percentages of CD3(+), CD4(+), and CD8(+) cells.
CONCLUSIONAdministration of α-GalCer after allogeneic bone marrow transplantation may promote immune reconstitution in the presence of aGVHD.
