Induction of endothelial cell apoptosis by anti-alpha-enolase antibody.
- Author:
Hong-bo YANG
1
;
Wen-jie ZHENG
;
Xuan ZHANG
;
Fu-lin TANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Apoptosis; drug effects; Autoantibodies; immunology; pharmacology; Autoimmune Diseases; immunology; Blotting, Western; Cell Line; Endothelial Cells; cytology; drug effects; Female; Flow Cytometry; Humans; Immunoblotting; Male; Middle Aged; Phosphopyruvate Hydratase; immunology; metabolism; Young Adult
- From: Chinese Medical Sciences Journal 2011;26(3):152-157
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo assess the prevalence of anti-alpha-enolase antibody in systemic autoimmune diseases in Chinese patients and its role in endothelial cell apoptosis.
METHODSThe reactivity of anti-alpha-enolase antibody in a variety of autoimmune disorders in Chinese patients was evaluated by dot blot assay. Endothelial cell apoptosis was investigated by in vitro incubation of endothelial cells with IgG purified from anti-alpha-enolase antibody-positive sera, with or without pre-incubation with recombinant alpha-enolase.
RESULTSAnti-alpha-enolase antibody was prevalent in different systemic autoimmune diseases with relatively high reactivity in Chinese patients. In vitro incubation of endothelial cells with IgG containing anti-alpha-enolase antibody induced apoptosis in a time- and dose-dependent manner. Apoptosis was partly inhibited by pre-incubation of the endothelial cells with recombinant alpha-enolase.
CONCLUSIONSOur data suggest that alpha-enolase is a common auto-antigen recognized by anti-endothelial cell antibodies in connective tissue disease. Interaction between alpha-enolase and its autoantibody plays a role in endothelial cell apoptosis. Changes other than cell killing may contribute to the pathogenesis of endothelial damage and microvascular lesions.