Combination of direct-ELISA and PCR for the rapid detection and identification of Salmonella spp.
- Author:
Jin-Lin HUANG
1
;
Xin-An JIAO
;
Zhi-Ming PAN
;
Qi-Yi WEN
;
Lin SUN
;
Xiu-Fan LIU
Author Information
- Publication Type:Journal Article
- MeSH: Enzyme-Linked Immunosorbent Assay; methods; Feces; microbiology; Humans; Polymerase Chain Reaction; methods; Salmonella; classification; genetics; isolation & purification; Serotyping
- From: Chinese Journal of Preventive Medicine 2004;38(5):331-334
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a protocol for the rapid detection of Salmonellae.
METHODSA mono-antibody-based direct-ELISA and PCR methods for the detection of Salmonella were developed previously. This study assessed the accuracy of both direct-ELISA and PCR methods for the rapid detection of Salmonella and set up a new detection protocol.
RESULTSThe sensitivity of the PCR method was higher than that of direct-ELISA method. In the 2002 spring physical examination for employees, 1 546 human fecal samples were examined by the combination of direct-ELISA and PCR method. Compared with the results of national standard method, the sensitivity and specificity of direct ELISA was 100% and 97.14%, respectively, while those of PCR method reached both 100%. It also indicated that combination use of two methods could give positive report within 40 hrs, and also achieve high sensitivity and specificity.
CONCLUSIONSBased on the results obtained, a protocol for the rapid detection of Salmonella was developed. The first step is to us direct-ELISA method to screen the large number of samples, and then use PCR method to validate the ELISA positive samples, and the final step is, if needed, is to use the national standard method to determine the serotypes of Salmonellae.
