Expression of hormone-sensitive lipase mRNA in diet-induced obesity resistant rats.
- Author:
Chang-Hao SUN
1
;
Rong LIU
;
Ying LI
;
Shu-Ran WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adipose Tissue; drug effects; enzymology; metabolism; Animals; Body Weight; drug effects; Dietary Fats; administration & dosage; Male; Obesity; enzymology; etiology; genetics; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Rats, Sprague-Dawley; Sterol Esterase; biosynthesis; genetics
- From: Chinese Journal of Preventive Medicine 2004;38(5):339-341
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEIn order to explore the expression of hormone-sensitive lipase (HSL) mRNA in white adipose tissue of diet-induced obesity-resistant (DIO-R) rats.
METHODSFifty male Sprague-dawley (SD) rats were randomly divided into control group and high-fat group and they were fed with basic diet and high-fat diet respectively for 13 weeks. DIO-R and DIO rats were selected according to their body weight and the quantity of energy intake, then observing changing of the body fat content, the level of serum growth hormone was determined by radioimmunoassay and the level of the HSL mRNA in white adipose tissue was determined by RT-PCR.
RESULTSThe body fat content of DIO-R rats were lower than those of DIO rats (P < 0.05). The level of serum growth hormone of DIO-R rats were higher than those of DIO rats (P < 0.05). High fat diets increased the level of the HSL mRNA in DIO-R rats.
CONCLUSIONIncreasing expression of HSL in white adipose tissue may play a part of role in resisting diet-induced obesity of rats.
